Independent LED light source avoids cross color. High contrast, high brightness, no drift, and no cross color make it more suitable for specific fluorescence channels. Equipped with CHROMA filter narrowband filter, the minimum spectral band resolution reaches 10nm, which can efficiently separates different fluorescence channels to achieve large information density without affecting the quality of data.

Dhyana 400D high-sensitivity refrigeration scientific CMOS camera is equipped, low signal-to-noise ratio and high resolution, with a new 1.2 inch surface array scientific CMOS chip. The quantum efficiency at 600 nm is up to 80%. 6.5 μm pixel size and 2*2 pixel binning function realized by FPGA, suitable for low-light detection and imaging. The Dhyana 400D has excellent performance, with only 2 electrons of readout noise. The browsing speed is up to 35 frames/s at 4 megapixels. And the data transmission speed is up to 5Gb/s to provide strong support for users’ work.

The 26.5mm field of view keeps the image flat, decreasing shooting and exposure time. The LED light source ensures more than 20,000 hours work, long-lasting and simple to maintain. Non-contact Hall switches are used to power the light source, and wireless charging technology is introduced to save wiring, which boosts system stability.

The fluorescence image stitching algorithm based on high-precision direct position feedback is used to splice images to obtain seamless whole-slide images and improve browsing experience. Pupil function of visual image enhancement algorithm is applied, no need to correct slides. The optical field information is efficiently estimated based on the limited excitation light information. When the light field information does not change with the increase of new sample, the light field information must be the right one for the set of optical path, and the light field can be calibrated based on this information.